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KMID : 0358419940370102003
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Korean Journal of Obstetrics and Gynecology
1994 Volume.37 No. 10 p.2003 ~ p.2018
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A Study on the Ontogenesis of Gonadotropes and Changes in the Gonadotropes in Mice Pituiteries During Pregnancy
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Abstract
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This study was carried out to localize the FSH cells and LH cells in mice pituitaries, to investigate the ontogenesis of these cells, to classifity the FSH cells and LH cells, to detect the immunoreactive sites in these cells, and to see the
morphological change of these cells in the pituitaries of the female mice at electron microscopic levels.
Fifteen BALB/c female mice and 50 BALP/c fetal mice were used in this study.
The avidin-biotin peroxidase complex (ABC) method was used for the immunohistochemical study of fetal mice pituitaries and the immunogold method was used for the immunocytochemical study of fetal and adult mice pituitaries. FSH cells and LH cells
were
localized in the pars distalis of pituitaries of all mothers and some fetuses. FSH cells and LH cells were first detected on the 17th day of gestation at the light and electron microscopic levels. FSH cells and LH cells showed significant
increase
in
number during the latedevelopmental period by immunohistochemistry. Immunoracctive stainings were detect only in the secretory granules in FSH cells and LH cells at the electron microscopic level.
FSH cells and LH cells could be classified into Type I cells (which were round and contained evenly distributed large secretory granules). Type Ii cells (which were angular and contained loosely distributed, small and medium-sized granules) and
Type III
cells (which were angular and contained peripherally distributed small secretory granules) by their morphology. Type I FSH cells were abundant in midpregnancy and term. And Type III FSH cells increased in percentage on the 15th and 17th day of
gestation. Type I LH cells were abundant in midpregnancy, and Type III LH cells were abundant in late pregnancy. Type II FSH cells and Type II LH cells showed no significant changes in percentage during pregnancy.
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KEYWORD
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